近日，我院巴基斯坦籍留学生Muhammad Rashid在中国农业科学院兰州兽医研究所殷宏研究员的指导下，于中科院JCR医学大类2区期刊《蜱与蜱传疾病》Ticks and Tick-borne Disease (IF=3.055)发表第一作者文章。殷宏研究员团队成功建立了环形泰勒虫转化的牛B淋巴细胞系并对其细胞因子产生的变化进行了研究。该细胞系将有助于环形泰勒虫致弱虫株疫苗的研发，用于环形泰勒虫病的预防。基于该系列研究，Rashid同学还在Genes(IF=3.331)和Parasitology(IF=2.456)另外发表了两篇第一作者文章。

Recently, the research team of Prof Yin Hong, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, established a Theileria annulata (T. annulata) transformed B cell line and found out the change of cytokines production of B cells after transformation. This cell line might be further processed for attenuation for vaccine production against theileriosis. The research findings were published in the international journals of “Genes” (IF=3.331), “Tick and Tick-borne Diseases” (CAS-Q2, IF=3.055) and “Parasitology” (IF=2.456).

   夏季，乡村养殖业受蜱叮咬的影响，会导致产奶量和体重降低。而蜱传病造成的经济影响取决于动物的感染率和死亡率。已报道的环形泰勒虫转化细胞系多是单核细胞混合细胞系，而由混合细胞系制备的致弱疫苗产生保护力并不能很好地预防泰勒虫病。因此，我们建立了环形泰勒虫转化的B细胞单克隆细胞系，并对该细胞系细胞因子的产生进行了定量PCR检测。

During summer, tick infestation in the country side was main hurdle for livestock productivity with respect to decrease milk production and weight loss. The economic importance of tick-borne diseases are due to morbidity and mortality. The already established T. annulata-transformed cell line was originated from a mixture of mononuclear cells. This attenuated mononuclear cell line was not so efficient. Hence, a single cell line originated from immune cells (B cells) was established. The cytokines production of this cell line was quantified by qPCR.

实验证明，转化的DC细胞可通过MHC I 和MHC II 分子传递给CD4+ 和CD8+ T细胞。并通过细胞流式技术对共培养后两种T细胞的增殖率的变化进行了检测。此外，通过定量PCR方法对GTPase基因的转录水平进行了检测分析，该基因在抗原递呈过程中发挥重要作用。

This intake antigen was presented via MHC class (I and II) molecules to co-cultured (CD4+ and CD8+) cells. The proliferation rate was measured by flow cytometry. The expression of small GTPase (Rab family genes) were quantified by qPCR which has important role for antigen cross-presentation.

原文链接Original links:

https://doi.org/10.1016/j.ttbdis.2019.101365 Ticks and Tick-borne Diseases

https://doi.org/10.3390/genes10050329 Genes

https://doi.org/10.1017/S0031182019001227 Parasitology

相关研究受到973项目（No.2015CB150300）、国家自然基金（No.31402189）、中国农业科学院创新工程（CAAS-ASTIP-2016-LVRI）、国家肉牛牦牛体系（CARS-37）和江苏省动物重要疫病与人兽共患病防控协同创新中心等项目的资助。

    This study was financially supported by the 973 Program (No. 2015CB150300); NSFC (No. 31402189); ASTIP (CAAS-ASTIP-2016-LVRI); NBCIS (CARS-37); Jiangsu Co-innovation Center programme for Prevention and Control of Important Animal Infectious Disease and Zoonosis, China.

Muhammad Rashid，巴基斯坦人，为我院2016级秋季学期预防兽医学专业博士研究生。2016年9月，在中国政府奖学金的资助下，加入我院兰州兽医研究所外寄生虫与虫媒疫病团队。该生博士期间以第一作者共发表了4篇SCI论文，如Genes(IF=3.331)、Parasitology(IF=2.456)、Ticks and Tick-borne Diseases(CAS-Q2, IF=3.055)和Poultry Science(CAS-Q2 Top, IF=2.216)，累计影响因子11.058。

Dr. Muhammad Rashid was a Pakistani scholar graduated from Graduate School of Chinese Academy of Agricultural Sciences(GSCAAS), who completed PhD in Preventive Veterinary Science (Sep, 2016-July, 2019) from the Lanzhou Veterinary Research Institute, CAAS, Beijing, China. He published his research findings in 4 well reputed SCI journals, such as Genes(IF=3.331), Parasitology (IF=2.456), Tick and Tick borne Diseases (CAS-Q2, IF=3.055) and Poultry Science (CAS-Q2 Top, IF=2.216) with a total Impact Factor of 11.058.

Dr. Muhammad Rashid’s publication list

https://www.researchgate.net/profile/Muhammad_Rashid65/research?ev=brs_act

Prof. Hong Yin’s publication list 

https://www.researchgate.net/profile/Hong_Yin5/research

Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (LVRI, CAAS)

http://lvri.caas.cn/en/