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《分子遗传学专题》之五

 时间:周五(1026日)13:30  地点:一教

题目:植物同源染色体重组分子机理

授课人简介:程祝宽 男,博士,1966年5月生。研究员,博士生导师。

   1987年获扬州大学农学系农学学士,1990年获扬州大学农学系作物遗传育种硕士,1999年获中国科学院遗传研究所理学博士,1999年至2002年在美国Wisconsin-Madison大学从事博士后研究。2002年12月入选中国科学院“百人计划”,终期考核为优秀。2003年获国家杰出青年基金资助,现为Chromosome Research杂志编委。主要研究内容包括:

1.植物减数分裂过程的遗传控制

  减数分裂是配子形成过程中进行的一种特殊分裂方式,其特点是染色体复制一次,细胞分裂两次,形成了染色体数目减半的配子。雌雄配子受精形成合子,染色体又恢复到原来的数目。由于减数分裂过程中来自父母双方染色体的充分重组和精确分离,既保证物种遗传物质的相对稳定,也为有性后代提供丰富的遗传多样性。减数分裂过程是一极其复杂的生命过程,涉及减数分裂的启动,同源染色体的配对、联会、交换和分离等一系列染色体的变化过程,这些过程受许多基因的调控,一直成为生物科学研究的热点。我们以水稻作为模式生物,通过正向和反向两种途径,系统研究参与减数分裂过程的基因,了解它们的作用网络,为最终解析减数分裂调控的分子机理提供依据。

 

2.水稻花器官发育及种子形成的分子机理

  花器官是植物特有的生殖器官,其遗传与发育受到许多基因的调控。水稻作为单子叶分子生物学研究的模式生物,其花器官与双子叶植物有着质的差别。我们以水稻为模式生物,通过诱发花器官变异的突变体,克隆相关基因,为深入了解单子叶生物花器官形成与发育的分子机理提供证据。

减数分裂研究(责任作者)


1. Hong LL, Tang D, Zhu KM, Wang KJ, Li M, and Cheng ZK (2012). Somatic and reproductive cell development in rice anther is regulated by a putative Glutaredoxin. Plant Cell 24: DOI:10.​1105/​tpc.​111.​093740.


2. Shen Y, Tang D, Wang KJ, Wang M, Huang J, Luo WX, Luo Q, Hong LL, Li M, and Cheng ZK (2012). The role of ZIP4 in homologous chromosome synapsis and crossover formation in rice meiosis. Journal of Cell Science DOI:10.1242/jcs.090993.


3. Che LX, Tang D, Wang KJ, Wang M, Zhu KM, Yu HX, Gu MH, Cheng ZK (2011). OsAM1 is required for leptotene-zygotene transition in rice. Cell Research 21:654-665.


4. Wang KJ, Wang M, Tang D, Shen Y, Qin BX, Li M, Cheng ZK (2011). PAIR3, an axis-associated protein, is essential for the recruitment of recombination elements onto meiotic chromosomes in rice. MBC 22: 12-19.


5. Wang M, Tang D, Wang KJ, Shen Y, Qin BX, Miao CB, Li, M, Cheng ZK (2011).OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis. The Plant Journal 67: 583-594.


6. Shao T, Tang D, Wang KJ, Wang M, Che LX, Qin BX, Yu HX, Li M, Gu MH and Cheng ZK (2011). OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis. Plant Physiol. 156: 1386-1396.


7. Wang M, Wang KJ, Tang D, Wei CX, Li M, Shen Y, Chi ZC, Gu MH, Cheng ZK (2010). The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice. Plant Cell 22: 417-430.


9. Wang KJ, Tang D, Wang M, Lu JF, Yu HX, Liu JF, Qian BX,Gong ZY, Wang X, Chen JM, Gu MH, Cheng ZK (2009). MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice. Journal of Cell Science 122: 2055-2063.


其它研究(责任作者)


10. Hong LL, Qian Q, Tang D, Wang KJ, Li M, and Cheng ZK (2012) A mutation in the rice chalcone isomerase gene causes the golden hull and internode1 phenotype. Planta, 2012, DOI 10.1007/s00425-012-1598.


11. Li M, Tang D, Wang KJ, Wu XR, Lu LL, Yu HX, Gu MH, Yan CJ and Cheng ZK (2011). Mutations in the F-box gene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice. Plant Biotechnology Journal 9: 2001-2012.


12. Qin BX, Tang D, Huang J, Li M, Wu XR, Lu LL, Wang KJ, Yu HX, Chen JM, Gu MH and Cheng ZK (2011). Rice OsGL1-1 is involved in leaf cuticular wax and cuticle membrane. Molecular Plant 4: 985-995.


13. Wang KJ, Tang D, Hong LL, Xu WY, Huang J, Li M, Gu MH, Xue YB, and Cheng ZK (2010). DEP and AFO regulate reproductive habit in rice. PLoS Genet 6: e1000818.


14. Zhu KM, Tang D, Yan CJ, Chi ZC, Yu HX, Chen JM, Liang JS, Gu MH, Cheng ZK (2010). ERECT PANICLE2 encodes a novel protein that regulates panicle erectness in indica rice. Genetics 184: 343-350.


15. Li M, Xiong GY, Li R, Cui JJ, Tang D, Zhang BC, Pauly M, Cheng ZK and Zhou YH (2009). Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth. The Plant Journal 60: 1055-1069.


16. Huang J, Zhang KW, Shen Y, Huang ZJ, Li M, Tang D, Gu MH, Cheng ZK (2009). Identification of a high frequency transposon induced by tissue culture, nDaiZ, a member of the hAT family in rice. Genomics 93: 274-281.


17. Zhang DF, Yang QY, Ding Y, Cao XF, Xue YB, Cheng ZK (2008) Cytological Characterization of the Tandem Repetitive Sequences and Their Methylation Status in the Antirrhinum majus Genome. Genomics 92: 107–114.


18. Zhang KW, Qian Q, Huang ZJ, Wang YQ, Li M, Hong LL, Zheng DL, Gu MH, Chu CC, and Cheng ZK (2006). GOLD HULL AND INTERNODE2 (GH2) encodes a primarily multifunctional cinnamyl-alcohol dehydrogenase (CAD) in Oryza sativa. Plant Physiol. 140: 972-983.


19. Bao WD, Zhang WL, Yang QY, Zhang Y, Han B, Gu MH, Xue YB, and Cheng ZK (2006). Diversity of centromeric repeats in two closely related wild rice species, O. officinalis and O. rhizomatis. Mol. Gen. Genomics 275: 421-430.


20. Zhang WL, Yi CD, Bao WD, Liu B, Cui JJ, Yu HX, Cao XF, Gu MH, Liu M, and Cheng ZK (2005). The transcribed 165bp CentO satellite is the major functional centromeric element in the wild rice species Oryza punctata. Plant Physilol. 139: 306-315.


21. Zhang DF, Yang QY, Bao WD, Zhang Y, Han B, Xue YB and Cheng ZK (2005). Molecular cytogenetic characterization of the Antirrhinum majus genome. Genetics 169: 325-335.


22. Hong LL, Qian Q, Zhu KM, Tang D, Huang ZJ, Gao L, Li M, Gu MH, Cheng ZK (2010). ELE restrains empty glumes from developing into lemmas. J. Genet. Genomics 37: 101-114.


23. Huang J, Tang D, Shen Y, Qin BX, Hong LL, You AQ, Li M, Wang X, Yu HX, Gu M, Cheng ZK (2010). Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.). J. Genet. Genomics 37: 23-36.


24. Yu HX, Wang X, Gong ZY, Tang D, Gu MH, Cheng ZK (2008) Generating of rice OsCENH3-GFP transgenic plants and their genetic applications. Chinese Science Bulletin 53: 2981-2988.


25. Cui JJ, Fan SC, Shao T, Huang ZJ, Zheng DL, Tang D, Li M, Qian Q, Cheng ZK (2007). Characterization and fine mapping of the ibf mutant in rice. J. Integr. Plant Biol. 49: 678−685.


26. Tang XM, Bao WD, Zhang WL, Cheng ZK (2007). Identification of chromosomes from multiple rice genomes using a universal molecular cytogenetic marker system. J. Integr. Plant Biol. 49: 953−960.


合作论文


27. Xu CH, Cheng ZK, and Yu WC(2012) Construction of rice minichromosomes by telomere mediated chromosomal truncation The Plant Journal DOI:10.1111/j.1365-313X.2012.04916.


28. Li J, Jiang JF, Qian Q, Xu YY, Zhang C, Xiao J, Du C, Luo W, Zou GX, Chen ML, Huang YQ, Feng YQ, Cheng ZK, Yuan M, Chong K (2011). Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation. Plant Cell 23: 628-640.


29. Wang GX, He QY, Liu F, Cheng ZK, Talbert PB. Jin WW (2011). Characterization of CENH3 proteins and centromere-associated DNA sequences in diploid and allotetraploid Brassica species. Chromosoma 120: 353–365.


30. Gong ZY, Liu XX, Tang D, Yu HX, Yi CD, Cheng ZK, Gu MH (2011). Non-homologous chromosome pairing and crossover formation in haploid rice meiosis. Chromosoma 120: 47–60.


31. Chai CL, Fang J, LiuY. Tong HN, Gong Y, Liu M, Wang YH, Qian Q. Cheng ZK and Chu CC (2011). ZEBRA2, encoding a carotenoid isomerase, is involved in photoprotection in rice. Plant Mol. Biol. 75: 211-221.


32. Lu F, Ammiraju J.S.S., Sanyal A., Zhang SL, Song RT, Chen JF, Li GS, Sui Y, Song X, Cheng ZK, de Oliveira A.C., Bennetzen J.L., Jackson S.A., Wing R.A., Chen MS (2009). Comparative sequence analysis of MONOCULM1-orthologous regions in 14 Oryza genomes. PNAS 106: 2071-2076


33. Gao DY, Gill N, Kim HR, Walling JG, Zhang WL, Fan CZ, Yu YS, Ma JX, Miguel PS, Jiang N, Cheng ZK, Wing RA, Jiang JM and Jackson SA (2009). A lineage-specific centromere retro-transposon in Oryza brachyantha. The Plant Journal 60: 820-831


34. Shang JJ, Tao Y, Chen XW, Zou Y, Lei CL, Wang J, Li XB, Zhao XF, Zhang MJ, Lu ZK, Xu JC, Cheng ZK, Wan JM and Zhu LH (2009). Identification of a new rice blast resistance gene, Pid3, by genome wide comparison of paired-nucleotide-bindingsite–Leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes. Genetics 182: 1303-1311


35. Cheng XD, Zhang DF, Cheng ZK, Keller B, Ling HQ (2009). A new family of Ty1-copia-Like retrotransposons originated in the tomato genome by a recent horizontal transfer event. Genetics 181: 1183-1193


36. Xue YB, Zhang YJ, Yang QY, Li Q, Cheng ZK, Dickinson, HG (2009). Genetic features of a pollen-part mutation suggest an inhibitory role for the Antirrhinum pollen self-incompatibility determinant. Plant Mol. Biol. 70: 499-509


37. Mueller L. A., et al. (2009). A Snapshot of the Emerging Tomato Genome Sequence. Plant Genome 2: 78-92


38. Chen SH, Yang Y, Shi WW, Ji Q, He F, Zhang ZD, Cheng ZK, Liu XN, Xu ML (2008). Badh2, encoding betaine aldehyde dehydrogenase, inhibits the biosynthesis of 2-acetyl-1-pyrroline, a major component in rice fragrance. Plant Cell 20: 1850-1861


39. Yan S, Yan CJ, Zeng XH, Yang YC, Fang YW, Tian CY, Sun YW, Cheng ZK, Gu MH (2008). ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice. Plant Mol. Biol. 68: 239–250


40. Fang J, Chai CL, Qian Q, Li CL, Tang JY, Sun L, Huang ZJ, Guo XL, Sun CH, Liu M, Zhang Y, Lu QT, Wang YQ, Lu CM, Han B, Chen F, Cheng ZK, Chu CC (2008). Mutations of genes in synthesis of the carotenoid precursors of ABA lead to preharvest sprouting and photo-oxidation in rice. The Plant Journal 54: 177-189


41. Ding Y, Wang X, Su L, Zhai JX, Cao SY, Zhang DF, Liu CY, Bi YP, Qian Q, Cheng ZK, Chu CC, Cao XF (2007). SDG714, a Histone H3K9 methyltransferase, is involved in Tos17 DNA methylation and transposition in rice. Plant Cell 19: 9-22


42. Yang QY, Zhang DF, Li Q, Cheng ZK, Xue YB (2007). Heterochromatic and Genetic Features are Consistent with Recombination Suppression of The Self-Incompatibility Locus in Antirrhinum. The Plant Journal 51: 140–151


43. Tang YL, Wen XG, Lu QT, Yang ZP, Cheng ZK, Lu CM (2007). Heat stress induces an aggregation of the light-harvesting complex of photosystem II in spinach plants. Plant Physiol. 143: 629-638


44. Jiang L, Zhang W, Xia Z, Jiang G, Qian Q, Li A, Cheng ZK, Zhu L, Mao L, Zhai W. A paracentric inversion suppresses genetic recombination at the FON3 locus with breakpoints corresponding to sequence gaps on rice chromosome 11L. Mol. Gen. Genomics 2007, 277: 263-272


45. Li L, Wang XF, Stolc V, Li XY, Zhang DF, Su N, Tongprasit W, Li SG, Cheng ZK, Wang J andDeng XW (2006). Genome-wide transcription analyses in rice using tiling microarrays. Nature Genetics 38: 124 – 129


46. Wang Y, Tang XM, Cheng ZK, Mueller L, Giovannoni J and Tanksley SD (2006) Euchromatin and pericentromeric heterochromatin: Comparative composition in the tomato genome.Genetics 172: 2529-2540


47. Mueller L. A., et al. (2005) The Tomato Sequencing Project, the first cornerstone of the International Solanaceae Project (SOL). Comparative and Functional Genomics 6: 153–158.


48. Jiao YL, Jia PX, Wang XF, Su N, Yu SL, Zhang DF, Ma LG, Feng Q, Jin ZQ, Li L, Xue YB, Cheng ZK, Zhao HY, Han B, and Deng XW (2005). A Tiling Microarray Expression Analysis of Rice Chromosome 4 Suggests a Chromosome-Level Regulation of Transcription. Plant Cell 17: 1641-1657


49. Lee HR, Zhang WL, Langdon T, Jin WW, Yan HH, Cheng ZK, and Jiang JM (2005). ChIP cloning reveals rapid evolutionary patterns of centromeric DNA in Oryza species. PNAS 102: 11793-11798


50. Nagaki K, Neumann P, Zhang DF, Ouyang S, Buell CR, Cheng ZK, and Jiang JM (2005). Structure, divergence, and distribution of the CRR centromeric retrotransposon family in rice. Molecular Biology and Evolution 22: 845-855


51. Guyot R, Cheng XD, Su Y, Cheng ZK, Schlagenhauf E, Keller B and Ling HQ (2005). Complex Organization and Evolution of the Tomato, Pericentromeric Region at the FER Gene Locus1. Plant Physilol. 138: 1205-1215


52. Nagaki K., Cheng, ZK, Ouyang S., Talbert P.B., Kim M., Jones K.M., Henikoff S., Buell C.R., Jiang JM (2004). Sequencing of a rice centromere uncovers active genes. Nature Genet. 36: 138-145


53. Zhang Y, Huang YC, Zhang L, Li Y, Lu TT, Lu YQ, Feng Q, Zhao Q, Cheng ZK, Xue YB, Rod A. Wing and Han B (2004). Structural features of the rice chromosome 4 centromere. Nucleic Acids Research 32: 2023-2030


54. Yu YS et al., (2003). In-depth view of structure, activity, and evolution of rice chromosome 10. Science, 300: 1566-1569.


55. Liu DF, Cheng ZK, Liu GQ, Liu GZ, Wang Y, Zhao XF, Zhu LH (2003). Characterization and mapping of a lesion mimic mutant in rice (Oryza sativa L.), Chinese Science Bulletin 48(9): 892-896.