中国农业科学院研究生院

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《分子遗传学专题》之四

2012-09-29 08:38:01 来源：作者：Admin 管理员：浏览量：

【打印】【纠错】

时间：周五（10月12日）13:30 地点：一教

题目：Systemin/jasmonate- mediated defense signaling in tomato

授课人：李传友研究员中国科学院微生物研究所

主讲人简介：

李传友，博士，研究员，博士生导师。

1991年山东农业大学学士；1994年山东农业大学硕士；1999年中国科学院遗传研究所博士。1999年至2003年，MSU-DOE Plant Research Laboratory博士后。2003年中国科学院“百人计划”入选者，2004年国家杰出青年科学基金获得者。李传友博士的主要研究方向是植物激素茉莉酸的生理功能及其作用机理。

1.以番茄为模式系统研究植物对昆虫抗性反应的分子机理

图1，番茄茉莉酸合成突变体spr2丧失了对昆虫的抗性

以番茄为模式系统，以经典的蛋白酶抑制剂为抗性标记基因，通过筛选抗性缺失突变体鉴定并分离植物对昆虫的抗性反应信号传导途径中的重要基因。目前已筛选到的番茄突变体主要分为两类：一类对系统素的识别发生了缺陷；另一类是JA的生物合成或信号传导发生了缺陷。采用图位克隆的方法成功地从番茄基因组中分离了控制JA合成的两个基因Spr2和JL1，目前正在进行生物学功能的研究。以茉莉酸合成突变体spr2（图1）和茉莉酸识别突变体jai1为材料，采用嫁接实验证明，在植物系统性抗性反应中进行长距离运输的信号分子是JA而不是传统认为的系统素，系统素的作用在于调控JA的生物合成。这是对本领域现有工作模型的重要修正。过量表达系统素前体基因（Prosystemin）的转基因番茄（35S::PS）组成型地表达高水平的抗性相关蛋白，表明在35S::PS植物中受伤反应始终处于开启的状态。目前我们正在以35S::PS为基础材料大规模筛选系统素/茉莉酸途径的抑制子突变。对更多突变体的表型分析、相应基因的分离和功能研究将会为最终阐明植物对昆虫抗性反应的遗传机理奠定基础。在此基础上通过对抗性信号传导途径中关键基因的遗传工程操作提高植物对昆虫的抗性。该项研究的长远目标是建立一种依靠植物自身的抗性因而是对环境友好的、可持续的控制农业害虫的策略。

2．番茄3号染色体测序及功能基因组学研究

作为重要的模式经济作物，番茄基因组较小，遗传学基础雄厚，并且具有许多拟南芥、水稻等模式植物所不具备的生物学现象（如果实的发育、成熟过程等），其功能基因组学的研究将为其它蔬菜、水果等重要经济作物的同类研究提供理论和方法上的直接借鉴。我们和其他科学家一道参加国际茄科基因组计划并负责番茄第3号染色体的序列分析（图2）、注释和比较基因组学研究。同时从番茄突变体材料入手，克隆重要经济性状相关基因并进行功能研究。

图2，番茄3号染色体遗传图、物理图及DNA序列的整合

3．以拟南芥为模式研究茉莉酸与其它激素互作调控植物生长发育和抗逆反应的分子机制

茉莉酸在植物的生长发育和抗逆反应两个方面都具有重要调控作用。我们的研究表明茉莉酸的这些生理功能是通过与其它植物激素的相互作用实现的。我们以拟南芥根系发育为模式研究茉莉酸与生长素互作协调植物的生长发育和抗性反应的分子基础（图3）。

图3，拟南芥酪氨酸硫基转移酶TPST调控生长素介导的干细胞转录因子PLT的表达，进而在植物根尖干细胞维持中起重要作用。

KEY PUBLICATIONS:

2012:

Chen R, Jiang H, Li L,Zhai Q, Qi L, Zhou W, Liu X,Li H, Zheng W, Sun J and Li C. (2012). The Arabidopsis Mediator subunit MED25 differentially regulates jasmonate and ABA signalings through interacting with MYC2 and ABI5. The Plant Cell. 24, 2898-2916.

The Tomato Genome Consortium (2012). The tomato genome sequence provides insights into fleshy fruit evolution. Nature.Volume: 485, Pages: 635-641. (Cover story)

Ren J, Li C-B and Li C. (2012). Tomato genome gets fully decoded--Paves way to tastier and healthier fruits. J. Genetics & Genomics. doi.org/10.1016/j.jgg.2012.06.001

Sun J, Qi L, Li Y, Chu J, and Li C. (2012). PIF4-mediated activation of YUCCA8 expression integrates temperature into the auxin pathway in regulating Arabidopsis hypocotyl growth. PLoS Genetics. 8(3), e1002594 (Featured Research, Faculty of 1000 Biology Evaluation)

Qi L, Yan J, Li Y, Jiang H, Sun J, Chen Q, Li H, Chu J, Yan C, Sun X, Yu Y, Li C-B and Li C. (2012). Arabidopsis plants differentially modulate auxin biosynthesis and transport during defense responses to the necrotrophic pathogen Alternaria brassicicola. New Phytologist. 195: 872-882.

Guo H, Kang L, Li C, Ren Q, Sun Y, Wang C, and Ge F (2012). Elevated CO2 reduces the resistance and tolerance of tomato plants to Helicoverpa armigera by suppressing the JA signaling pathway. PLoS One. 7(7), e41426.

2011：

Chen Q, Sun J,Zhai Q,Zhou W, Qi L, Xu L, Wang B, Chen R, Jiang H, Qi J, Li X, Palme K, and Li C. (2011).The basic helix-loop-helix transcription factor MYC2 directly represses PLETHORA expression during jasmonate-mediated modulation of the root stem cell niche in Arabidopsis. The Plant Cell. 23, 3335-3352. (Faculty of 1000 Biology Evaluation)

Chen M, Liu H, Kong J, Yang Y, Zhang N, Li R, Yue J, Huang J, Li C, Cheung A,and Tao L. (2011). RopGEF7 regulates PLETHORA-dependent maintenance of the root stem cell niche in Arabidopsis. The Plant Cell. 23, 2880-2894.

Sun J, Chen Q, Qi L, Jiang H, Li S, Xu, Y, Liu F, Zhou W, Pan J, Li X, Palme K and Li C (2011). Jasmonate modulates endocytosis and plasma membrane accumulation of the Arabidopsis PIN2 protein. New Phytologist. 191, 360-375. (Faculty of 1000 Biology Evaluation)

Li H, Jiang H, Bu Q, Zhao Q, Sun J, Xie Q, and Li C (2011). The Arabidopsis RING finger E3 ligase RHA2b acts additively with RHA2a in regulating ABA signaling and drought response. Plant Physiology. 156, 550-563.

Sun J, Jiang H and Li C (2011). Systemin/jasmonate-mediated systemic defense signaling in tomato. Molecular Plant. 4, 607-615.

Zhang L, Jia C, Liu L, Zhang Z, Li C and Wang Q (2012). The involvement of jasmonates and ethylene in Alternaria alternata f. sp. lycopersici toxin-induced tomato cell death. Journal of Experimental Botany. 62, 5405-5418.

Sun Y, Yin J, Cao H, Li C, Kang L, Ge F. (2011). Elevated CO2 influences nematode-induced defense responses of tomato genotypes differing in the JA pathway. PLoS ONE. 6(5), e19751.

2010：

Zhou W, Wei L, Xu J, Zhai Q, Jiang H, Chen R, Chen Q, Sun J, Chu J, Zhu L, Liu C-M and Li C (2010). Arabidopsis tyrosylprotein sulfotransferase acts in the auxin/PLETHORA pathway in regulating postembryonic maintenance of the root stem cell niche. The Plant Cell. 22, 3692-3709.

Wei J, Wang L, Zhao J, Li C, Ge F and Kang L. (2010). Ecological trade-offs between jasmonic acid-dependent direct and indirect plant defences in tritrophic interactions. New Phytologist. 189, 557-567.

Li C and Li J (2010). Toward understanding the molecular mechanisms governing plant hormone actions: A brief introduction to the Major Research Program “Molecular mechanisms of plant hormone actions” funded by the National Natural Science Foundation of China (NSFC). Chinese Science Bulletin. 55, 2197

Liu F, Jiang H, Ye S, Chen W-P2, Liang W, Xu Y, Sun B, Sun J, Wang Q, Cohen JD and Li C (2010). The Arabidopsis P450 protein CYP82C2 modulates jasmonate-induced root growth inhibition, defense gene expression and indole glucosinolate biosynthesis. Cell Research. 20, 539-552.

2009：

Sun J,Xu Y, Ye S, Jiang H, Chen Q, Liu F,Zhou W, Chen R, Li X,Tietz O, Wu X, Cohen J,Palme K and Li C (2009). Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation. The Plant Cell. 21, 1495-1511.

Mueller LA et al., (2009). A snapshot of the emerging tomato genome sequence. The Plant Genome. 2, 78-92.

Jiang H, Li H, Bu Q and Li C (2009). The RHA2a-interacting proteins ANAC019 and ANAC055 may play a dual role in regulating ABA response and jasmonate response. Plant Signaling & Behavior. 4:5, 464-466.

Bu Q, Li H, Zhao Q, Jiang H, Zhai Q, Zhang J, Wu X, Sun J, Xie Q, Wang D and Li C (2009). The Arabidopsis RING finger E3 ligase RHA2a is a novel positive regulator of ABA signaling during seed germination and early seedling development. Plant Physiology. 150, 463-481.

Liang W, Li C-B, Liu F, Jiang H, Li S, Sun J, Wu X, Li C (2009). The Arabidopsis homologs of CCR4-associated factor 1 exhibit mRNA deadenylation activity and play a role in plant defense responses. Cell Research. 19, 307-316.

2008：

Li C-B, Zhao J, Jiang H, Geng Y, Dai Y, Fan H, Zhang D, Chen J, Lu F, Shi J, Sun S, Chen J, Yan X, Lu C, Chen M, Cheng Z, Ling H, Wang Y, Xue Y, Li C (2008). A snapshot of the Chinese SOL Project. Journal of Genomics and Genetics. 35, 387-390.

Qi J, Qian Q, Bu Q, Li S, Chen Q, Sun J, Liang W, Zhou Y, Chu C, Li X, Ren F, Palme K, Zhao B, Chen J, Chen M, Li C (2008). Mutation of the rice NARROW LEAF1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport. Plant Physiology. 147, 1947-1959.

Bu Q, Jiang H, Li C-B, Zhai Q, Zhang J, Wu X, Sun J, Xie Q and Li C (2008). Role of the Arabidopsis thaliana NAC transcription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses. Cell Research. 18, 756-767.

2007：

Li H, Sun J, Xu Y, Jiang H, Wu X, and Li C (2007). The bHLH-type transcription factor AtAIB positively regulates ABA response in Arabidopsis. Plant Molecular Biology. 65, 655-665.

Sun J, Jiang H, Xu Y, Li H, Wu X, Xie Q and Li C (2007). The CCCH-type zinc finger proteins AtSZF1 and AtSZF2 regulate salt stress responses in Arabidopsis. Plant & Cell Physiology. 48, 1148-1158.

Zhai Q, Li C-B, Zheng W, Wu X, Zhao J, Zhou G, Jiang H, Sun J, Lou Y, and Li C (2007). Phytochrome chromophore deficiency leads to overproduction of jasmonic acid and elevated expression of jasmonate-responsive genes in Arabidopsis. Plant & Cell Physiology. 48, 1061-1071.

2006：

Zheng W, Zhai Q, Sun J, Li C-B, Zhang L, Li H, Zhang X, Li S, Xu Y, Jiang H, Wu X and Li C (2006). Bestatin, an inhibitor of aminopeptidases, provides a chemical genetics approach to dissect jasmonate signaling in Arabidopsis. Plant Physiology. 141, 1400-1413

Li C-B, Zhao J, Jiang H, Wu X, Sun J, Zhang C, Wang , Lou Y and Li C (2006). The wound-response mutant suppressor of prosystemin-mediated responses6 (spr6) is a weak allele of the tomato homolog of CORONATINE-INSENSITIVE1 (COI1). Plant & Cell Physiology. 47, 653-663

Li C-B, Sun J, Jiang H, Wu X, and Li C (2006). Systemic defense signaling in tomato. Chinese Science Bulletin. 50, 1817-1822

Canoles MA, Beaudry RM, Li C and Howe GA (2006). Deficiency of linolenic acid in lefad7 mutant tomato changes the volatile profile and sensory perception of disrupted leaf and fruit tissue. J. Amer. Soc. Hort. Sci. 131, 284-289

2005以前：

Mueller LA, Tanksley SD, Giovannoni JJ, van Eck J, Stack S, Choi D, Kim BD, Chen M, Cheng Z, Li C, Ling H, Xue Y, Seymour G, Bishop G, Bryan G, Sharma R, Khurana J, Tyagi A, Chattopadhyay D, Singh NK, Stiekema W, Lindhout P, Jesse T, Lankhorst RK, Bouzayen M, Shibata, D, Tabata S, Granell A, Botella MA, Giuliano G, Frusciante L, Causse M and Zamir D. (2005). The Tomato Sequencing Project, the first cornerstone of the International Solanaceae Project (SOL). Comparative and Functional Genomics. 6, 153-158

Li C, Schilmiller AL, Liu G, Lee GI, Jayanty S, Sageman C, Vrebalov J, Giovannoni JJ, Yagi K, Kobayashi Y and Howe GA (2005). Role of β-oxidation in jasmonate biosynthesis and systemic wound signaling in tomato. The Plant Cell. 17, 971-986

Li C, Liu G, Xu C, Lee G, Bauer P, Ganal M, Ling H and Howe GA (2003). The tomato Suppressor of prosystemin-mediated response2 gene encodes a fatty acid desaturase required for the biosynthesis of jasmonic acid and the production of a systemic wound signal for defense gene expression. The Plant Cell. 15, 1646-1661

Li L*, Li C*, Lee GI and Howe GA (2002). Distinct roles for jasmonate synthesis and action in the systemic wound response of tomato. Proc Natl Acad Sci USA. 99, 6416-6421 (* These authors contributed equally to this work)

Li C, Williams MM, Loh Y-T, Lee GI and Howe GA (2002). Resistance of cultivated tomato to cell content-feeding herbivores is regulated by the octadecanoid-signaling pathway. Plant Physiology. 130, 494-503

Li L, Li C and Howe GA (2001). Genetic analysis of wound signaling in tomato: evidence for a dual role of jasmonic acid in defense and female fertility. Plant Physiology. 127, 1414-1417